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Cytotoxic molecule mRNA expression in chronically rejected human kidney allografts.

TitleCytotoxic molecule mRNA expression in chronically rejected human kidney allografts.
Publication TypeJournal Article
Year of Publication2005
AuthorsNocera, A, Tagliamacco, A, Ferrante, A, Fontana, I, Rolla, D, De Palma, R, Del Galdo, F, Ginevri, F, Barocci, S, Valente, U
JournalTransplant Proc
Date Published2005 Jul-Aug
KeywordsAntigens, CD, Base Sequence, Chronic Disease, Cytokines, DNA Primers, Graft Rejection, Granzymes, Humans, Interleukins, Kidney Transplantation, RNA, Messenger, Serine Endopeptidases, T-Lymphocytes, Cytotoxic, Transplantation, Homologous

The pathogenesis of immunological and nonimmunological components that cause chronic kidney allograft nephropathy (CAN), is not yet completely understood. To explore the possible contribution of alloreactive cytotoxic T cells, we analyzed the transcription of cytotoxic molecules such as granzyme B and perforin using semiquantitative RT-PCR on surgically removed grafts obtained from two groups: group 1 (n = 10) were cases of CAN; group 2 (n = 3) had no CAN. Among group 1 kidneys, granzyme-B was expressed in 7 of 10, whereas perforin was detectable in 9 of 10 cases; their detection was not related to the presence of superimposed signs of acute graft lesions. Cytotoxic molecules were never found in group 2 kidneys. These results show that explanted chronically rejected grafts display cytotoxic molecule transcripts in addition to Th2 type cytokines, such as IL-10, IL-3, and IL-6, suggesting that both cellular and humoral alloreactive mechanisms may play important roles in CAN pathogenesis.

Alternate JournalTransplant. Proc.
PubMed ID16182715
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