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H4N8 subtype avian influenza virus isolated from shorebirds contains a unique PB1 gene and causes severe respiratory disease in mice

TitleH4N8 subtype avian influenza virus isolated from shorebirds contains a unique PB1 gene and causes severe respiratory disease in mice
Publication TypeJournal Article
Year of Publication2012
AuthorsBui, VN, Ogawa, H, , , Karibe, K, Matsuo, K, Award, SSA, Minougou, GL, Yoden, S, Haneda, H, Ngo, LH, Tamaki, S, Yamaoto, Y, Nakamura, K, Saito, K, Watanabe, Y, Runstadler, J, Huettmann, F, Happ, GM, Imai, K
JournalVirology
Volume423
Pagination77–88
Abstract

H4N8 subtype avian influenza viruses were isolated from shorebirds in eastern Hokkaido. All the isolatesshared >99.7% nucleotide homology, and all the viral genes except for PB1 were highly related to those ofA/red-necked stint/Australia/1/04. Thus, the isolates were regarded as PB1 reassortants. The most similarPB1 gene was identi&\#64257;ed in A/mallard/New Zealand/1615-17/04 (H4N6) with nucleotide homology of90.9%. BALB/c mice intranasally inoculated with the H4N8 isolates developed severe respiratory disease,which eventually led to death in some mice. The virus was isolated from the lungs, and viral antigen wasdetected in the lungs with pneumonia. Other H4 subtype viruses tested did not cause any symptoms inmice, although these viruses were also isolated from the lungs. The PB2 gene of the H4N8 isolates containsK482R, but not the E627K or D701N substitutions. The PB1-F2 gene of the isolates consists of a 101-aminoacid unique sequence, but lacks the N66S mutation

DOI10.1016/j.virol.2011.11.019
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